Module A: Molecular Biology

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INSTRUMENTATION UV/visible spectrophotometer, laminar air flow, microscope, pH meter, autoclave, centrifuges, shakers, water purification system, auto pipettes, weighing balances, Electrophoresis apparatus, Transilluminater, PCR etc.

Introduction to recombinant DNA tools and genetic engineering techniques

General rules to perform molecular biology experiments

Preparation of solutions for the molecular biology experiments.

Isolation of plasmid, genomic, metagenomic DNA.

Agrose gel electrophoresis of isolated DNA.

Spectrophotometeric estimation of quantity and purity of isolated DNA.

Restriction digestion of DNA.

Ligation of fragmented DNA in the plasmid.