Accutase Solution

Additional Details

Accutase is a cell detachment solution which has been developed to meet the most demanding requirements for gentle and effective detachment of adherent cells. Cell membranes and surface epitopes will not be harmed and the structural and functional quality of the surface proteins remains completely intact. The combination of protease and collagenolytic activities maximizes its versatility for cell detachment and tissue dissociation. Effectivity has been proven in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines and insect cells. Accutase does not contain mammalian or bacterial derived products. So there is no contamination risk for your cell culture. Accutase performs exceptionally well in detaching cells for: Routine cell passage assays Migratin- and proliferation assays Analysis of cell surface markers Virus growth assays FACS analyses Production scale-up (bioreactor) Features: Gently detaches adherent cells in minutes Effective for most cell culture applications For tissue dissociation for primary cell culture Maximum cell viability and plating efficiency Protection of surface epitopes Non-mammalian origin No neutralization required Easy to use and cost effective Product Specification: pH 6.8 – 7.8 Osmolality 270 – 320 mOsmol/kg Endotoxin = 10 EU/ml Cell Detachment tested Sterility tested Storage = -15°C Solvent PBS with 0.5 mM EDTA