Bio Globus

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General Mycoplasma, first discovered in 1898, were classified as viruses. There are more than 100 known species belonging to the genus Mycoplasma. They are the smallest free-living, self-replicating organisms and lack a cell wall so that antibiotics such as penicillin which interfere with murein formation are not effective. If not treated mycoplasma contaminations can lead to diminished cell growth and to the loss of cultures. MycoKill AB is active at low concentrations for a broad range of mycoplasma subspecies. It acts on the protein synthesis machinery by interfering with ribosome translation as well as with mycoplasma RNA transcription. Mycoplasma Effect cell growth Modulate cytokine production of cells Influence virus propagation in cell culture Induce chromosomal aberrations in vitro Effect DNA/RNA synthesis Reduce metabolic activation Detection of Mycoplasma It is necessary to screen cell cultures periodically for a possible mycoplasma contamination. To ensure detection it is recommended that different methods are used. The detection is necessary when working with established cell lines as well as with primary material. Applications Mycoplasma infected established cell lines Decontamination of primary material Prophylaxis for cell culture Features Effective mycoplasma removal Effective against a broad range of mycoplasma Does not negatively effect eukaryotic cell proliferation Easy to use Cost effective. Working Concentration Recommended final concentration: 20 ml/L Product Specifications pH 4.0 – 4.5 Osmolality 280 – 340 mOsmol/kg Endotoxin ≤ 10 EU/ml Cell Culture tested Sterility tested Storage ≤ -15°C Solvent PBS